Archives of Clinical and Biomedical Research

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2572-5017

Abstracting and Indexing

Review ArticleOpen Access

Electric Phenomenon in Bones as a Result of Piezoelectricity of Hydroxyapatite

Maciej Pawlikowski

AGH University Science and Technology, Cath. Mineralogy, Petrography and Geochemistry, al. Mickiewicza 30,

30-049 Kraków, Poland

*Corresponding  Author:  AGH  University  Science  and  Technology,  Cath.  Mineralogy,  Petrography  and

Geochemistry, al. Mickiewicza 30, 30-049 Kraków, Poland

Received: 08 May 2017; Accepted: 12 May 2017; Published: 15 May 2017


Abstract:

Conducted biomineralogical studies indicate piezoelectric properties of apatite, which is one of the major components of bone. This publication presents hypothetical correlations between piezoelectric properties of bone apatite and the accompanying electromagnetic phenomena in bones and their environment. It notes the correlations between electrical properties of bones and changes in the electromagnetic field around bones, as well as connections between external pressure changes etc. and electrical phenomena in the bones.


Keywords:

Apatite Piezoelectricity; Bone; Electromagnetic Phenomena

1. Introduction

The first research data regarding piezoelectricity was the result of many years of work by the Curie brothers [1-10]. It applied to electrical phenomena occurring in quartz crystals under mechanical pressure. These studies have been extended to other minerals and synthetics, showing the presence of similar phenomena in them.

Particularly interesting was the study of electrical phenomena in the bones. Those piezoelectric phenomena have long been of interest both in biochemical and electrical terms [11-23] as well as in a practical sense, mainly in relation to bone healing and acceleration of fracture unions [24-28]. The influence of electrical phenomena in the bones on the functioning of bone elements, including bone cell morphology, has also been observed [29-30].

Recent studies have shown that bone piezoelectricity may be associated with the piezoelectric properties of bone hydroxyapatite [18, 22-23]. Those studies have become the basis for the considerations presented in this publication. It attempts to explain the relationship between the piezoelectricity of bone hydroxyapatite and the piezoelectricity of the entire bone. The interpretation of the described phenomena has also been discussed in the context of the potential relationship of bone piezoelectricity to other phenomena. Research on the piezoelectric properties of bones opens new prospects for understanding many biochemical phenomena in bones and other organs [31].

2. Study Conducted with the Author's Own Resources

Considering the impact of bones on the functioning of the body, we need to take into account their huge surface: after converting the surface of all bone trabeculae from all human bones to one surface, we get the value of 380 000 to 420 000 m2  [18]. On that surface, elements of bone such as bone marrow, body fluids, cells and others get in contact with bone collagen containing micro-crystals of hydroxyapatite. On this contact point, there is constant physicochemical equilibrium between the "biological world" and the "mineral world" of bone. On such a vast surface, elements are passed both ways throughout a human’s life, and in the course of life, this system of minerals vs. "biology" is evolving.

All biological processes occurring in the bones are accompanied by electrical phenomena connected, among others, with their piezoelectric properties. Their relevance seems to be difficult to overestimate.

Piezoelectric testing of bone hydroxyapatite is not possible at the present apparatus level due to the nano-size of its crystals. As a result, piezoelectricity of this mineral was studied on large crystals (Figure 1A) and the obtained results can be transferred to nano-crystals in bones. Likewise, electrical phenomena observed in large crystals of apatite can be transferred to nano-crystals found in bones (Figure 1 B).

image

Figure 1: A - Example of a hydroxyapatite plate cut perpendicularly to the optical axis Z after dusting with gold. The dark edge is a paraffin border; after dusting with gold and removal of the border, upper and lower surfaces of the plates were electrically separated. B - Example of the currents chart recorded during a hand movement [22].

When discussing piezoelectricity, we need to pay attention to the details of the relation between collagen and bone hydroxyapatite, as well as electrical phenomena manifesting outside the bone.

image

Figure 1: A- Potential site for the transfer of electrical charges from the mechanically excited apatite crystal to the collagen fiber found in the bone structure. B - visualization of the induction of electrical charges in a mechanically deformed  plate  crystal  of  apatite.  C-  schematic  of  induction  of  electrical  charges  in  mechanically  deformed collagen-apatite fiber (trabecula).

image

Figure 2:  Distribution of electrical charges in bones [19] A - long bone, bent; B - long bone; A, D - at rest; B –weight-bearing; C – not weight-bearing.

While discussing the above phenomena, we should address some of the problems that may be related to them, such as:
- Mechanical bone stress and osteoporosis,

- Influence on the functioning of certain tissues and organs

- External relations between pressure, gravity and electromagnetic field, and electrical phenomena occurring in bones
- Mechanical phenomena occurring in bones vs. external electromagnetic field.

- Influence of piezoelectric phenomena on fracture healing.

3. Mechanical Bone Stress and Osteoporosis

Generated "bone" currents can also affect bone collagen itself, especially the binding between collagen and hydroxyapatite. It appears that currents generated during stress (during walking) promote stability of these bonds. Absence of these currents can probably cause the bindings (ionic: apatite - collagen) to weaken or be broken. The effect is loosening and weakening of the bone structure.

This situation takes place in bone unaffected by gravity, eg. in astronauts in space. Studies show that osteoporosis in conditions where gravity exists is an effect of clogging of micro-arteries in the bones. Crystallizing substances in those arteries are both phosphates and cholesterol [21,23].

Piezoelectrically induced currents in bone apatite may affect various bone components, including arteries in which endothelial cells have a polar structure (Figure 3).

image

This issue is complex and not fully explained, and it requires further experimental research. It seems that treating astronauts’ bones in space with weak alternating currents could probably minimize the osteoporosis process.

 

Figure  3:  Sketch  of  the  theoretical  influence  of  currents  generated  in  bone  on  the  structure  of  destroyed phospholipid with polar structure of endothelial cell membrane. A- phospholipid with polar structure of endothelial

cell membrane. B- structure of partially destroyed phospholipid with endothelial cell membrane (eg. due to inflammation in the body). The site of destruction may be deprived of electrical charges by flowing "bone" currents. This way, the possibility of using damage site as the center of calcification or cholesterol crystallization is reduced.

4. Influence on the Functioning of Certain Tissue and Organs

The  bone  system:  trabeculae  –  bone  marrow  and  other  elements  (vessels,  nerves,  etc.)  is  a  system  of physicochemical equilibrium. It changes throughout life, from youth to old age. Its function is to transfer elements from the "biological part" to the "mineral part" and vice versa [18]. So far, electrical phenomena generated in bones in the "biological part" have not been studied.

Their influence seems unquestionable as the vast majority of chemical processes that take place in bones, including their "biological part", are ionic. It is also likely that the microcurrents that form in the bones stimulate the synthesis of bone-generated blood components.

5.  External  Relations  Between  Pressure,  Gravity  and  Electromagnetic  Field,  and  Electrical

Phenomena Occurring in Bones

Mechanically bent apatite plates generate differences of potentials at the ends of the crystals [22]. Undoubtedly, they do not cover all the problems that may be associated with piezoelectric phenomena observed in bones. Thus, walking that exerts pressure on the (leg) bones and on the hydroxyapatite microcrystals in trabeculae causes the creation of currents [12]. They are alternating currents in the up-down - up-down direction [11, 19].

It is assumed that generating currents in bone may also occur as a result of changes in external atmospheric pressure, as well as changes in gravity (eg. in astronauts). It can not be ruled out that changes in the electromagnetic field also cause micro-vibrations of bone apatite crystals and generation of currents (Figure 4). The significance of these theoretically discussed phenomena is difficult to overestimate.

image

Figure 4: Schematic diagram of the influence of external pressure, gravitation on electrical phenomena in bones. A - example of pressure changes (ζp) on the human femur. B - schematic of generating alternating currents in collagen fiber by hydroxyapatite crystals subjected to pressure by external forces

6. Mechanical Phenomena Occurring in Bones Vs. External Electromagnetic Field

Although the currents generated in a single micro-crystal of bone hydroxyapatite are negligible, the combination of currents generated by moist collagen fibers may probably add up [14]. A result of this phenomenon may be stronger currents and higher voltages at the ends of the collagen fibers, in whole trabeculae, and even on the surface of bone [28].

Because stressing and unstressing of bones leads to the development of alternating currents, walking causes the same flow of currents [29, 19]. Currents generated in this way undoubtedly influence the creation of a weak, alternating electromagnetic field around the bone that generates these currents. It is also an alternating field, and its value depends not only on the value of the generated currents, but also on the enormous surface area of the bone (the sum of the surfaces of all the trabeculae generating currents). It may be too daring to say that the bone that generates alternating currents can be treated like a great transceiver antenna, but it may actually be so.

image

Figure 5: Diagram of the mechanism of generating an alternating electromagnetic field by the femur stressed during walking.  A-  femur  under  alternating  pressure  (ζp)  during  walking.  B-  image  of  a  collagen  fiber  generating alternating  currents  through  deformed  hydroxyapatite  crystals.  C-  hypothetical  image  of  electromagnetic  field
changes around (AE) under the influence of alternating currents generated in the bone while walking.

7. Influence of Piezoelectric Phenomena on Fracture Healing

Complexity of the bone healing process is obvious. Among its elements are the generation of collagen with centers of apatite crystallization, the phenomena of angiogenesis (Pawlikowski - Biomineralogy of angiogenesis - in print), the re-launch of alkaline phosphatase synthesis, and others.

After a bone fracture, various components of bone are destroyed, including collagen fibers mineralized with apatite in damaged trabeculae. For this reason, as well as through the stabilization of the fracture (plaster fixation, AO plates, etc.), electrical systems occurring in the unbroken, stressed bone get damaged and disturbed. Hence, the use of artificial - external alternating electromagnetic field may stimulate bone healing by approximating the "electrical conditions" occurring in bones to natural conditions. This alternating field may also probably stimulate angiogenesis and other processes. Although some publications suggest the toxicity of the alternating electromagnetic field used to accelerate bone healing [27], it seems very important in this case to match the generated field to the field produced by the natural bone. It requires further research; however, at the current stage of knowledge it is purely theoretical.

Summary

Despite the fact that the currents in bones are very weak (as they are in the brain), they are absolutely elementary. Their incompletely recognized role for the functioning of the whole body is difficult to overestimate.

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